Achieve consistent bioprocessing with TRIS AMINO buffers and derivatives from ANGUS Chemical Company

Spectrum Chemical is proud to offer a curated portfolio of TRIS buffers from ANGUS Chemical Company. From early-stage biopharmaceutical development through commercialization, ANGUS’ high-purity, cGMP-compliant TRIS AMINO buffers and TRIS derivatives help the world’s leading biopharmaceutical companies achieve the most consistent, cost-effective manufacturing processes. The company innovated the Tromethamine molecule more than 70 years ago, proudly building the TRIS brand to represent a strong history of quality and reliability. ANGUS remains the world’s largest and only full integrated manufacturer of TRIS tromethamine buffers, providing 100% traceability and secure, sustainable supply.

 TRIS AMINO™ AC Ultra Pure

Less Clumping. More Preparation Efficiency.

While most TRIS buffers are prone to clumping and hardness, the next-generation TRIS AMINO™ AC buffers feature a unique crystal morphology that results in less time, energy and cost to break up clumps prior to buffer preparation.

TRIS AMINO AC uses a proprietary process technology that produces a larger, more rounded particle. Without making any changes to the molecule itself, this process helps prevent the formation of strong, hard cakes even when subjected to a variety of packaging, shipping and storage conditions.

  • Low interparticle cohesion between crystals requires less energy to break up clumps
  • More spherical crystals for easy dispensing and transport through conveyors
  • Fewer fine particles for lower dust emissions
  • No additives or anti-clumping agents
  • Highest purity and testing requirements including bioburden and endotoxin
  • cGMP-compliant with comprehensive validation documentation
  • Proven safe use in commercial drug products
  • Fully traceable supply chain with line of sight to the base materials
  • Elemental impurity risk assessment using ICP-MS technology
  • Ability to maintain pH from 7 to 9 by utilizing a combination of free base and its salts
  • Amine and alcohol functionality to provide versatile chemical reactivity

Typical Properties*



Molecular weight 


Melting point 


pKa @ 20°C


pKa @ 37°C




pH of 0.1M aqueous solution (20°C)


Δ pH/10X dilution

-0.05 to -0.10

*Values shown are typical properties and are not to be considered product specifications. Test methods available upon request.

An Innovative Solution to a Long-standing Industry Problem

Clumping during transport and storage is a well-known and challenging characteristic of conventional TRIS buffer products. To address this long-standing industry challenge, ANGUS Chemical Company developed the next generation TRIS AMINO™ AC Advanced Crystals, produced in our newly expanded, state-of-the art manufacturing facility in Ibbenbüren, Germany.

Conventional TRIS crystal structure*

TRIS AMINO AC Advanced Crystals

Larger Size, Fewer Fine Particles, Smoother Surface = Less Clumping. The unique morphology of TRIS AMINO AC buffers helps reduce cake strength, resulting in less clumping and hardness. 4x magnification at 2 cm observation distance

Uses and Applications

  • Pharmaceutical raw material compliant with USP, EP, JPC, and ChP monographs
  • Commercial purification (downstream processing) of biological molecules including monoclonal antibodies and recombinant proteins
  • Processing aid for insulin manufacturing
  • Non-sterile pharmaceutical excipient
  • Buffer for molecular biology and clinical diagnostic applications
  • pH buffering of enzymes in biotechnology applications
  • Small mlecule API salt to systematically adjust drug hydrophilicity
  • Small molecule synthesis building block

Chemical Name: Tris(hydroxymethyl)aminomethane (2-amino-2-(hydroxymethyl)-1,3-propanediol)

Common Synonyms: TRIS, Tromethamine, Trometamol, THAM, Tris base, TRIZMA®

Product Information

Chemical Type Chemical Name CAS No. Catalog No. Size


TRIS AMINO AC Ultra Pure USP, EP Biologics Grade



5 KG


12 KG


25 KG

BIS-TRIS Biologics Grade

Exceptional Purity. Diverse Applications.

BIS-TRIS Biologics Grade buffer from ANGUS Chemical Company is a 99.0% pure buffering agent widely used as a diagnostic reagent and in gel electrophoresis separations, cell culture and bioprocessing applications. BIS-TRIS is ideal for a variety of biological-based formulations and nucleic acid systems and is known to complex with various metals.

  • High purity, 99.0%
  • Produced under a single global quality system that includes ISO 9001:2015
  • Proven safe use in commercial drug products
  • Highly soluble in water
  • Ideal for use as an alternative to cacodylate, maleic acid and citrate

 Uses and Applications

  • Purification of biological molecules (downstream processing)
  • Raw materials for API/drug substance formulation
  • Cell culture buffer
  • Fractionation of hemoglobin
  • Gel electrophoresis
  • Diagnostics reagents
  • Research and development buffer (R&D)
  • Analytical chromatography
  • Non-sterile excipient

Typical Properties*



Molecular weight 


Melting point 

100.0 –104.0°C

pH (1.0M aqueous solution @25°C):

9.5 – 11.0

Working pH Range:

5.8 – 7.2

pKa @ 20°C:

6.35 – 6.65

*Values shown are typical properties and are not to be considered product specifications. Test methods available upon request.

ANGUS, TRIS AMIONO and DISCOVER A BETTER WAY are trademarks of ANGUS Chemical Company.

Chemical Name: Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

Common Synonyms: BIS TRIS, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol

Product Information

Chemical Type Chemical Name CAS No. Catalog No. Size


BIS-TRIS Biologics Grade



5 KG


12 KG


25 KG


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